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KMID : 0613820080180010052
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Journal of Life Science
2008 Volume.18 No. 1 p.52 ~ p.57
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Optimal Production of Xylooligosaccharide by Using Recombinant Endoxylanase from Bacillus subtilis
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Kim Yeun-Hee
Heo Sun-Yeon
Kim Mi-Jin
Lee Jae-Hyung
Kim Young-Man
Nam Soo-Wan
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Abstract
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Xylan is a major hemicellulose component of the cell walls of monocots and hardwood, representing up to 30% of the dry weight of these plants. To efficiently hydrolyze xylan, the endoxylanase gene from Bacillus sp. was expressed in B. subtilis DB431 by introducing the plasmid pJHKJ4. The total activity of the recombinant endoxylanase reached about 857 unit/ml by batch fermentation of B. subtilis DB431/pJHKJ4 in LB maltose medium. The majority (>92%) of endoxylanase was efficiently secreted into the culture medium. The recombinant endoxylanase hydrolyzed more the birchwood xylan efficiently than the other xylans. When 4 % concentration of xylan was used, the highest production of xylooligosaccharide was observed, and xylobiose and xylotriose were the major products. Optimal amount of enzyme and reaction time for producing xylooligosaccharide were found to be 10 unit and 1 hr, respectively. In addition, the temperature of 40’c~50’c gave the highest production of xylooligosaccharide. Consequently, the optimized conditions for the production of xylooligosaccharide through the hydrolysis of xylan were determined as follows: 10 unit endoxylanase,50’c , 4% birchwood xylan, 1 hr reaction.
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KEYWORD
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Bacillus subtilis, endoxylanase, optimization, xylan, xylooligosaccharide
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